GmR

BBa_P1012 Version 1

Component

Source: http://parts.igem.org/Part:BBa_P1012

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tom Knight

Date created: 2006-03-15 12:00:00

Date modified: 2015-05-08 01:14:12

gentamycin/kanamycin resistance cassette

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_P1012_sequence (Version 1)

Description

The aacA-aphD gene from the transposon Tn4001 conveys resistance to both Gentamycin and Kanamycin with a single two-domain resistance protein.

Notes

;primers to make Biobrick version of GmR gene cassette (P1012)

gtttcttcgaattcgcggccgcttctagagTGGACTTGACTGAGTTTATGGAA, P3aph-F

gtttcttcctgcagcggccgctactagtaTCTGGACTTGACTCACTTCCTTT, P3aph-R


;P3aph-R (3' - 5')
;aaaggaagtgagtcaagtccagatactagtagcggccgctgcaggaagaaac

Source

Originally from Tn4001 transposon; sequence M18086. Created from plasmid pISM2062 from Chris Minion, Iowa State University.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
acetyl-transferase	81,411	CDS, feature/cds
aacA-aphD (GmR, KanR)	412,1851	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_P1012/1

Member of these Collections

• //chassis/prokaryote/mflorum
• //direction/forward
• //plasmidbackbone/component/selectionmarker/antibioticresistance
• iGEM Parts Registry