BBa_P1015

BBa_P1015 Version 1

Component

This part has been discontinued.

Source: http://parts.igem.org/Part:BBa_P1015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tom Knight

Date created: 2006-06-10 11:00:00

Date modified: 2015-05-08 01:14:12

Gentamicin resistance aph(2

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_P1015_sequence (Version 1)

Description

Novel gentamicin resistance gene. Derived from Genbank DQ208936, pTEX5500ts, kind gift of Barbara E. Murray. This, in turn was derived from Genbank AF016483. See pmid=16391062, pmid=9593155

Notes

XbaI site at 660 removed by mutating c->t which also improves codon usage. gatgatttttacagggatattttagagaacgaaatctac,P1014-XF
gtagatttcgttctctaaaatatccctgtaaaaatcatc,P1014-XR

Source

DQ208936 with mutations

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
aph(2 doubleprime)-ld	120,1025	feature/cds, CDS
Terminator	1040,1070	feature/stem_loop, stem_loop
-35, -10	37,73	promoter, feature/promoter

Other Properties

• igem#experience: None
• igem#sampleStatus: Discontinued
• igem#status: Deleted
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_P1015/1

Member of these Collections

• iGEM Parts Registry