Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14
promoter (lacI regulated)
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_R0010_sequence (Version 1) |
Description
Inverting regulatory region controlled by LacI ( The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor
Notes
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
Source
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.| Sequence Annotation | Location | Component / Role(s) |
| end of LacI coding region (inactive) BBa_R0010 CAP binding site -35 -10 LacI binding site start | 1,88 1,200 89,126 137,142 161,166 166,200 173,173 | feature/cds CDS engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter promoter feature/promoter feature/binding non_covalent_binding_site feature/start start_codon |