LacI

BBa_R0010 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0010
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14

promoter (lacI regulated)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0010_sequence (Version 1)

Description

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0011, etc.)

The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor BBa_C0010 which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows BBa_R0010 to be used as a inverter or as a detector of lactose or IPTG.

Notes

LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.

Source

The Plac insert was PCR'd from the MG1655 strain of E.coli K12.

Sequence Annotation Location Component / Role(s)
end of LacI coding region (inactive)
BBa_R0010
CAP binding site
-35
-10
LacI binding site
start
1,88
1,200
89,126
137,142
161,166
166,200
173,173
feature/cds CDS
engineered_region feature/BioBrick
non_covalent_binding_site feature/binding
promoter feature/promoter
promoter feature/promoter
feature/binding non_covalent_binding_site
feature/start start_codon
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_R0010/1