Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14
Promoter (lacI regulated, lambda pL hybrid)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_R0011_sequence (Version 1) |
Description
Inverting regulatory region controlled by LacI ( The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (
Notes
hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.
LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.