lacI+pL

BBa_R0011 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0011
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14

Promoter (lacI regulated, lambda pL hybrid)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_R0011_sequence (Version 1)

Description

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0011, etc.)

The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0010) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).

Notes

hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.

LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.

Source

represillator of Elowitz and Leibler (2000)

Sequence Annotation Location Component / Role(s)
lac O1
-35
lac O1
-10
BBa_R0011
3,19
20,25
26,42
43,48
1,54
operator feature/operator
feature/promoter promoter
feature/operator operator
promoter feature/promoter
feature/BioBrick engineered_region
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_R0011/1