cI lam

BBa_R0051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14

promoter (lambda cI regulated)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R0051_sequence (Version 1)

    Description

    The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).



    Notes

    In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)

    Incompatible with host expressing cI repressor.

    Source

    A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

    Sequence Annotation Location Component / Role(s)
    -10
    -35
    OR1
    OR2
    BBa_R0051
    		38,43
    15,20
    25,41
    1,17
    1,49
    		promoter feature/promoter
    feature/promoter promoter
    non_covalent_binding_site feature/binding
    feature/binding non_covalent_binding_site
    engineered_region feature/BioBrick

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0051/1
     

    Attachments

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