cI lam

BBa_R0051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:14

promoter (lambda cI regulated)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0051_sequence (Version 1)

Description

The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).



Notes

In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)

Incompatible with host expressing cI repressor.

Source

A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

Sequence Annotation Location Component / Role(s)
-10
-35
OR1
OR2
BBa_R0051
38,43
15,20
25,41
1,17
1,49
promoter feature/promoter
feature/promoter promoter
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
engineered_region feature/BioBrick
igem#experience Works  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_R0051/1