lux pR

BBa_R0062 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0062
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (luxR & HSL regulated -- lux pR)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_R0062_sequence (Version 1)

Description

Promoter activated by LuxR in concert with HSL

The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.

Notes

This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts BBa_R0062 and BBa_R0063 were derived is shown in the picture below.



Source

V. fischeri

Sequence Annotation Location Component / Role(s)
LuxR/HSL
-35
-10
start
BBa_R0062
1,20
20,25
42,47
53,53
1,55
feature/binding non_covalent_binding_site
promoter feature/promoter
promoter feature/promoter
feature/start start_codon
feature/BioBrick engineered_region
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_R0062/1