lux pL

BBa_R0063 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0063
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (luxR & HSL regulated -- lux pL)



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_R0063_sequence (Version 1)

Description

The lux cassette of V. fischeri contains a left and a right promoter. The left promoter gives weak constitutive expression of downstream genes.This expression is down-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription from Pr, repressing transcription from Pl



Notes

This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts BBa_R0062 and BBa_R0063 were derived is shown in the picture below.Includes most of Lux reulatory region, including the LuxR binding site which activates the right promoter. A putative LuxR autorepression binding site is also identified adjacent to the -10 site of the right promoter. This 2nd site has 55% identity with the first site. Putative inverted repeats (of size 18-27 bp) also exist between these two sites (not marked above), which may represent binding sites for other regulatory proteins.

Unspecified.

Source

V. fischeri.

Sequence Annotation Location Component / Role(s)
LuxR/HSL
-10
-35
start
Putative LuxR/HSL
BBa_R0063
1,20
115,122
89,94
128,128
130,149
1,151
non_covalent_binding_site feature/binding
feature/promoter promoter
feature/promoter promoter
feature/start start_codon
non_covalent_binding_site feature/binding
feature/BioBrick engineered_region
igem#experience None  
igem#partStatus Released HQ 2013  
igem#sampleStatus In stock  
igem#status Available  
synbiohub#ownedBy user/james  
synbiohub#ownedBy user/myers  
synbiohub#topLevel BBa_R0063/1