Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15
Promoter (lambda cI and luxR regulated -- hybrid)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_R0065_sequence (Version 1) |
Description
cI repressor negatively regulates this promoter and LuxR activates its transcription.The effect of cI is dominant over LuxR. This part is based on the LuxR and cI repressor regulated hybrid promoter designed and tested by Ron Weiss. It requires the binding of two cI repressor dimers for maximal repression and contains two cI repressor binding sites namely, OR1 and OR2. This promoter is leaky in the sense that 'some' transcription is seen in the absence of both cI and LuxR.
| LuxI | cI | activity of promoter |
| + | + | zero |
| + | - | maximum |
| - | + | zero |
| - | - | leaky (no quantitative information) |
Notes
This part was designed based on the LuxR and cI repressor regulated hybrid promoter tested by Ron Weiss and the LuxR-LuxICDABE sequence annotated by Tom Knight
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0065 Lux Box -10 OR2 cI lux p(R) start -35 OR1 cI -10 | 1,97 6,25 47,52 57,73 58,58 71,76 81,97 94,97 | engineered_region feature/BioBrick non_covalent_binding_site feature/binding promoter feature/promoter feature/binding non_covalent_binding_site promoter feature/promoter feature/promoter promoter non_covalent_binding_site feature/binding feature/promoter promoter |