RhlR+C4

BBa_R0071 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0071
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ronny Krashinsky
Date created: 2004-01-24 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (RhlR & C4-HSL regulated)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0071_sequence (Version 1)

Description

Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.


This is the natural sequence taken from Pseudomonas aeruginosa.


Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.

Notes

The -10 and -35 sites are labeled as identified in [Pearson97].


The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).

Source

Pseudomonas aeruginosa rhlAB promoter

Sequence Annotation Location Component / Role(s)
RhlR
-35
-10
start
1,20
19,24
42,47
53,53
non_covalent_binding_site feature/binding
feature/promoter promoter
promoter feature/promoter
start_codon feature/start
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R0071/1