OmpR

BBa_R0082 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0082
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (OmpR, positive)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R0082_sequence (Version 1)

    Description

    Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.

    Notes

    The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1.

    An alternate version, BBa_R0083, cuts out the C2 and C3 sites.

    The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.

    Source

    NC_000193 E. coli K12

    Sequence Annotation Location Component / Role(s)
    C1 OmpR
    C2 OmpR
    C3 OmpR
    -35
    -10
    		13,30
    34,51
    54,71
    75,80
    98,103
    		non_covalent_binding_site feature/binding
    feature/binding non_covalent_binding_site
    feature/binding non_covalent_binding_site
    feature/promoter promoter
    promoter feature/promoter

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0082/1
     

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