OmpR

BBa_R0082 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0082
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (OmpR, positive)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0082_sequence (Version 1)

Description

Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.

Notes

The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1.

An alternate version, BBa_R0083, cuts out the C2 and C3 sites.

The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.

Source

NC_000193 E. coli K12

Sequence Annotation Location Component / Role(s)
C1 OmpR
C2 OmpR
C3 OmpR
-35
-10
13,30
34,51
54,71
75,80
98,103
non_covalent_binding_site feature/binding
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/promoter promoter
promoter feature/promoter
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R0082/1