OmpR

BBa_R0083 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0083
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (OmpR, positive)



    • Details

    Types
    DnaRegion

    Roles
    promoter

    Regulatory

    Sequences BBa_R0083_sequence (Version 1)

    Description

    Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This is a truncated version of BBa_R0082.

    Notes

    The promoter deletes the C2 and C3 regions of BBa_R0082 (bases 37-74 in BBa_R0082) and inserts an 8-base linker before the -35 and -10 signal region. The efficacy of this promoter versus the full ompC upstream region (BBa_R0082) or the ompF upstream region (BBa_R0084) is currently unknown.
    The a at base 41 is mutated to t to avoid XbaI
    site. Speculation that this was a previous assembly site.

    Source

    NC_000193 E. coli K12

    Sequence Annotation Location Component / Role(s)
    C1 OmpR
    linker
    -35
    -10
    A
    		13,30
    37,44
    45,50
    68,73
    41,41
    		non_covalent_binding_site feature/binding
    feature/misc sequence_feature
    promoter feature/promoter
    feature/promoter promoter
    feature/s_mutation point_mutation

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0083/1
     

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