OmpR

BBa_R0084 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0084
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
Date created: 2004-01-27 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter (OmpR, positive)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R0084_sequence (Version 1)

Description

Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompF. Phosphorylated OmpR binds to the three operator sites and activates transcription.

Notes

The promoter includes three OmpR binding sites: F1, F2, and F3. The promoter starts at -107 and goes up to the transcriptional start site at +1. A distant fourth binding site, F4, has been deleted. It is involved in negative regulation of ompF (Head, Tardy and Kenney, 1998), so its deletion is intended to make the promoter solely activating. The efficacy of this promoter versus the ompC upstream region (BBa_R0082) or the truncated ompC upstream region (BBa_R0083) is currently unknown.

Source

NC_000913 E. coli K12

Sequence Annotation Location Component / Role(s)
F1 OmpR
F2 OmpR
F3 OmpR
-35
-10
11,28
31,48
51,68
73,78
88,93
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/binding non_covalent_binding_site
feature/promoter promoter
promoter feature/promoter
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R0084/1