BBa_R0184

BBa_R0184 Version 1

Component

Source: http://parts.igem.org/Part:BBa_R0184

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Bartholomew Canton

Date created: 2007-04-25 11:00:00

Date modified: 2015-05-08 01:14:15

T7 promoter (lacI repressible)

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_R0184_sequence (Version 1)

Description

A mutant version of the T7lac promoter designed by Dubendorff and Studier. The promoter is based on the strong ??10 T7 promoter but incorporates an A->C mutation at -16. The LacI operator site is a partially symmetric 25bp sequence.

Notes

The -16 mutation was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)

Source

This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -16.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0183 fragment	1,20	feature/promoter, promoter
lacI binding site	20,44	operator, feature/operator
Center of binding site symmetry	31,31	feature/misc, sequence_feature

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_R0184/1

Member of these Collections

• //chassis/bacteriophage/t7
• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/negative
• //rnap/bacteriophage/t7
• iGEM Parts Registry