Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Bartholomew Canton
Date created: 2008-04-07 11:00:00
Date modified: 2015-05-08 01:14:15
T7 promoter (lacI repressible)
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_R0185_sequence (Version 1) |
Description
A lac repressible T7 promoter. This promoter is similar to those found in the pET vectors with T7lac promoters. I mutated the -10 base to a G, which should make it about 4% of the strength of consensus. The base numbering is as used by Studier and coworkers (JMB 1991, 219, 45-59). See BBa_R0183 for more information.Notes
The -10 mutation from consensus was included to weaken the consensus promoter as described by Imburgio and co-workers (see BBa_R0183 for more information)Source
This sequence is based on the T7lac promoter described by Dubendorff and Studier (JMB 1991, 219, 45-59). The only modification is the mutation at -10.| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter sequence LacI binding site | 1,20 20,44 | feature/promoter promoter non_covalent_binding_site feature/binding |