cI lam

BBa_R1051 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R1051
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
Drew Endy
Date created: 2003-01-31 12:00:00
Date modified: 2015-05-08 01:14:15

Promoter, Standard (lambda cI regulated)



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_R1051_sequence (Version 1)

Description

[Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].

The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).



Notes

In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)

Modified to comply with BBa_S0001:
TTGACA-17N-GATACT

Incompatible with host expressing cI repressor.

Source

A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000

Sequence Annotation Location Component / Role(s)
-35
OR1
OR2
-10
BBa_R1051
15,20
25,41
1,17
38,43
1,49
feature/promoter promoter
promoter feature/promoter
promoter feature/promoter
promoter feature/promoter
engineered_region feature/BioBrick
igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
In stock
igem#status
Available
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_R1051/1