Types | DnaRegion
|
Roles | promoter
Regulatory
|
Sequences | BBa_R1051_sequence (Version 1)
|
Description
[Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].
The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor BBa_C0051. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
Notes
In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03)
Modified to comply with BBa_S0001:
TTGACA-17N-GATACT
Incompatible with host expressing cI repressor.