lacITst

BBa_S03469 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_S03469
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: ETH Zurich iGEM Team 2005
Date created: 2006-05-01 11:00:00
Date modified: 2015-05-08 01:14:23

Lambda-lacI input system TESTER (IPTG controlled lacI+pL efficiency)



    • Details

    Types
    DnaRegion

    Roles
    Measurement

    engineered_region

    Sequences BBa_S03469_sequence (Version 1)

    Description

    Constitutive (tetR) lacI-production (lacI+LVA) to block promoter (lacI+pL) in ground state of a 2-state system. lacI+pL can be activated, e.g. over IPTG, thus leading to the production of CFP (yellow fluorescence). This device allows to estimate repressed (lacI) activity/full activity under ITPG absence/presence.

    Notes

    For more details see Sensing Device and Event Processing Device in the ETH Team iGEM2005 WiKi documentation.

    Source

    Registry 7.05

    Access Instance Definition
    public
    public
    		BBa_S03334
    BBa_E0422
    		input-lacI
    BBa_E0422

    Sequence Annotation Location Component / Role(s)
    BBa_S03334
    BBa_E0422
    		1,1433
    1442,2358
    		input-lacI
    BBa_E0422

    igem#sampleStatus
    It's complicated
    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_S03469/1
     

    Attachments

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