Strep-tag

BBa_T2012 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_T2012
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2009-08-10 11:00:00
Date modified: 2015-05-08 01:14:52

Strep-tag tail domain (WRHPQFGG)



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_T2012_sequence (Version 1)

Description

This is a Strep tag tail domain designed to be at the C-terminus of a protein. Strep tags are a type of affinity tag that can be used to purify and/or measure the quantity of proteins.

Notes

This domain encodes the Strep tag sequence AWRHPQFGG. The codons at each amino acid position were selected to avoid rare codons as much as possible in organisms like E. coli, humans, mouse, B. subtilis, C. elegans, Streptomyces coelicolor and other common model organisms and/or industrial hosts. Another factor in codon selection was avoiding avoid repeat sequences.

Source

This is a synthetic sequence.

Sequence Annotation Location Component / Role(s)
Strep-tag
double stop codon
1,24
25,30
feature/tag tag
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_T2012/1