V5 tag

BBa_T2019 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_T2019
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty
Date created: 2009-09-02 11:00:00
Date modified: 2015-05-08 01:14:52

V5 epitope tag head domain (GKPIPNPLLGLDST)



Types
DnaRegion

Roles
polypeptide_domain

Protein_Domain

Sequences BBa_T2019_sequence (Version 1)

Description

This is a V5 epitope tag head domain designed to be at the N-terminus of a protein. V5 tags are a type of epitope tag that can be used to measure the quantity of proteins.

Notes

This head domain begins with Met-Lys to promote efficient translation of the protein in E. coli. Rare codons from various model organisms were avoided where possible.

Source

This is a synthetic sequence.

Sequence Annotation Location Component / Role(s)
start codon
preferred AAA +2 codon
V5 epitope tag
1,3
4,6
7,48
feature/start start_codon
feature/start start_codon
tag feature/tag
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_T2019/1