Types | DnaRegion
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Roles | chromosome
Cell
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Description
Taken from http://openwetware.org/wiki/E._coli_genotypes#HB101
F- mcrB mrr hsdS20(rB- mB-) recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20(SmR) glnV44 λ-
Please note that different sources have different genotypes so treat this information with caution.
* From a GIBCO BRL list of competent cells.
* Hybrid of E. coli K12 and E. coli B
* Host for pBR322 and many plasmids
* Sigma lists the deletion Δ(gpt,proA). Check this.
* Promega does not list F-, mcrB, or mrr
* Streptomycin resistant
* References:
o Boyer, H.W. and Roulland-Dussoix, D. (1969) J. Mol. Biol. 41, 459.
o Smith, M., Lorow, D., and Jessee, J. (1989) FOCUS 11, 56.
o Lacks S and Greenberg JR (1977) J Mol Biol 114:153.
Notes
None.
Source
Brown iGEM 2006 team