NEB 10b

BBa_V1024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_V1024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinoo Selvarajah
Date created: 2009-07-22 11:00:00
Date modified: 2015-05-08 01:14:53

E. coli strain NEB 10-beta



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NEB 10b
Types
DnaRegion

Roles
Cell

chromosome

Description

  1. Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
  2. Activity of nonspecific endonuclease I (endA) eliminated for highest quality plasmid preparations
  3. Resistance to phage T1 (fhuA2)
  4. Suitable for blue/white screening by α-complementation of the β-galactosidase gene
  5. Reduced recombination of cloned DNA (recA1)
  6. Clone large plasmids and BACs
  7. DH10B??? derivative
  8. Free of animal products
  9. K12 Strain


Source

araD139 ∆(ara-leu)7697 fhuA lacX74 galK (Φ80 ∆(lacZ)M15) mcrA galU recA1 endA1 nupG rpsL (StrR) ∆(mrr-hsdRMS-mcrBC)

igem#experience
Works
 
igem#sampleStatus
No part sequence
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_V1024/1