NEB 10b

BBa_V1024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_V1024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Vinoo Selvarajah
Date created: 2009-07-22 11:00:00
Date modified: 2015-05-08 01:14:53

E. coli strain NEB 10-beta



    • Details

    Types
    DnaRegion

    Roles
    Cell

    chromosome

    Description

    1. Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
    2. Activity of nonspecific endonuclease I (endA) eliminated for highest quality plasmid preparations
    3. Resistance to phage T1 (fhuA2)
    4. Suitable for blue/white screening by α-complementation of the β-galactosidase gene
    5. Reduced recombination of cloned DNA (recA1)
    6. Clone large plasmids and BACs
    7. DH10B??? derivative
    8. Free of animal products
    9. K12 Strain


    Source

    araD139 ∆(ara-leu)7697 fhuA lacX74 galK (Φ80 ∆(lacZ)M15) mcrA galU recA1 endA1 nupG rpsL (StrR) ∆(mrr-hsdRMS-mcrBC)

    igem#experience
    Works
     
    igem#sampleStatus
    No part sequence
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_V1024/1
     

    Attachments

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