Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sri Kosuri
Date created: 2006-04-24 11:00:00
Date modified: 2015-05-08 01:14:54
T7.2 gene 5
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_Z0121_sequence (Version 1) |
Description
T7 DNA Polymerase consists of two proteins, the gene 5 protein and the processivity factor E. coli thioredoxin. Thioredoxin forms a 1:1 complex with the T7 protein and increases its processivity by at least 1000-fold, the complex also increases the activity of the associated 5??->3?? exonuclease on dsDNA but has little effect on ssDNA exonucleolytic activity.| Sequence Annotation | Location | Component / Role(s) |
| atc...gag -> atc...gaa codon shuffle added pstI | 4,39 2116,2121 | point_mutation feature/s_mutation feature/mutation sequence_alteration |