Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Mallet
Date created: 2006-06-20 11:00:00
Date modified: 2015-05-08 01:14:55
T7.2 gene 1, codon-shuffled
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_Z0170_sequence (Version 1) |
Description
Encodes the same protein as T7.2 gene 1, with first 12 codons after start shuffled to reduce RBS-CDS secondary structure.Notes
The nucleotide sequence of the first 12 AA after the start codon were codon-shuffled to minimize the predicted RNA secondary structure that is formed between the RBS assigned to this gene in the T7.2 design and the coding sequence. The predicted dG of the RBS-CDS junction is -0.01 kCal/mol.Source
Based on gene 1 of T7.| Sequence Annotation | Location | Component / Role(s) |
| gttgac -> GTAGAT S1 -35 site gat -> GAC S1 -10 site S1 promoter startsite aac..gac -> aat..gat | 2497,2502 2498,2502 2518,2520 2520,2525 2532,2532 4,39 | feature/s_mutation point_mutation sequence_feature feature/misc point_mutation feature/s_mutation feature/promoter promoter feature/promoter promoter feature/s_mutation point_mutation |