Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Reshma Shetty & Tom Knight
Date created: 2004-05-03 11:00:00
Date modified: 2015-05-08 01:14:55
High copy BioBrick assembly plasmid
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | pSB1A3_sequence (Version 1) |
Description
pSB1A3-1 is a high copy number plasmid carrying ampicillin resistance.The replication origin is a pUC19-derived pMB1 (copy number of 100-300 per cell).
pSB1A3 has terminators bracketing its MCS which are designed to prevent transcription from *inside* the MCS from reading out into the vector. The efficiency of these terminators is known to be < 100%. Ideally we would construct a future set of terminators for bracketing a MCS that were 100% efficient in terminating both into and out of the MCS region.
A fully annotated Genbank file is attached below.
| Sequence Annotation | Location | Component / Role(s) |
| suffix E. coli his operon terminator terminator stem loop VR rep(pMB1) (rev) ORI ampR (rev) VF2 end of terminator terminator stem loop prefix | 1,21 22,93 30,64 157,176 261,875 276,276 1035,1895 2018,2037 2094,2097 2107,2126 2136,2157 | engineered_region feature/BioBrick feature/misc sequence_feature stem_loop feature/stem_loop primer_binding_site feature/primer_binding CDS feature/cds feature/misc sequence_feature CDS feature/cds primer_binding_site feature/primer_binding feature/misc sequence_feature feature/stem_loop stem_loop feature/BioBrick engineered_region |