BBa_K883703
BBa_K883703 Version 1 (Component)
IPTG induced promoter + GFP with a His-tag fused to a coiled coil

BBa_K1189016
BBa_K1189016 Version 1 (Component)
GFP with a K coil under the control of a lacI promoter

BBa_K883705
BBa_K883705 Version 1 (Component)
IPTG induced promoter + GFP with a His-tag fused to a coiled coil

BBa_M36918
BBa_M36918 Version 1 (Component)
T1 from E. coli rrnB

BBa_K1189020
BBa_K1189020 Version 1 (Component)
Light chain human ferritin with his-tag and E-coil under the lacI inducible promoter

BBa_K1189019
BBa_K1189019 Version 1 (Component)
Heavy chain human ferritin with his-tag and E-coil under the lacI inducible promoter

LacY
BBa_K079014 Version 1 (Component)
LacY transporter protein from E. coli

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_K2123301
BBa_K2123301 Version 1 (Component)
Novel Synthetic Phytochelatin codon optimized for E. coli expression

BBa_K1033204
BBa_K1033204 Version 1 (Component)
pSBLb4E15 E. coli and lactobacilli shuttle vector with erythromycin resistance

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.