BBa_K092500
BBa_K092500 Version 1 (Component)
TetR cds with RBS and Terminator + pTetR, RFP with RBS

BBa_K318501
BBa_K318501 Version 1 (Component)
lacI pL + RBS + RcsB + TT

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

BBa_I13006
BBa_I13006 Version 1 (Component)
Promoter R0040 w/ YFP (-LVA) TT

BBa_K415005
BBa_K415005 Version 1 (Component)
pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term

BBa_K199165
BBa_K199165 Version 1 (Component)
pT7 + RBS + GFP + TT in pSB3T5

BBa_K2066500
BBa_K2066500 Version 1 (Component)
UNS 2 Sequence, from Torella et al., 2013

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.