NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

BBa_M39009
BBa_M39009 Version 1 (Component)
NanT

BBa_M39008
BBa_M39008 Version 1 (Component)
NanR

NAND2616
BBa_I732301 Version 1 (Component)
NAND Candidate (U073O26D001O16)

NAND2717
BBa_I732302 Version 1 (Component)
NAND Candidate (U073O27D001O17)

BBa_K1969000
BBa_K1969000 Version 1 (Component)
Nano-lantern(cAMP-1.6)

NAND2-7
BBa_I732304 Version 1 (Component)
NAND Candidate (U073O22D001O47)

BBa_K737065
BBa_K737065 Version 1 (Component)
mutant Nanc

NAND01
BBa_I732303 Version 1 (Component)
NAND Candidate (U073O22D001O46)

NAND59
BBa_I732305 Version 1 (Component)
NAND Candidate (U073O22D059O46)

NANDbeta
BBa_I732306 Version 1 (Component)
NAND Candidate (U073O11D002O22)

BBa_K737063
BBa_K737063 Version 1 (Component)
truncated NanC transcript

BBa_M39007
BBa_M39007 Version 1 (Component)
Promoter Sequence for NanR

BBa_K1422502
BBa_K1422502 Version 1 (Component)
Zea mays Nicotianamine aminotransferase (corn NAAT)

BBa_K1969012
BBa_K1969012 Version 1 (Component)
Linker sequence between CUP1 promoter and Nano-lantern(cAMP-1.6)

BBa_K1969010
BBa_K1969010 Version 1 (Component)
Linker sequence between GAL1 promoter and Nano-lantern(cAMP-1.6)

BBa_K235028
BBa_K235028 Version 1 (Component)
[K235024][K235001] (NAND gate control test, positive control)

BBa_K1969014
BBa_K1969014 Version 1 (Component)
Nano-lantern(cAMP-1.6) regulated by CUP1 promoter

BBa_K1969008
BBa_K1969008 Version 1 (Component)
The linker sequence between ADH1 promoter and Nano-lantern(cAMP-1.6)

BBa_K1947020
BBa_K1947020 Version 1 (Component)
This polypeptide will be fused with the recombinant protein which we want to purify.

BBa_K1969007
BBa_K1969007 Version 1 (Component)
Nano-lantern(cAMP-1.6) regulated by ADH1 promoter

BBa_K1969013
BBa_K1969013 Version 1 (Component)
Nano-lantern(cAMP-1.6) regulated by GAL1 promoter

BBa_K235026
BBa_K235026 Version 1 (Component)
[K235022][K235001] (NAND gate control test, pLac positive control)

BBa_K235027
BBa_K235027 Version 1 (Component)
[K235024][K235000] (NAND gate control test, arabinose positive control)

BBa_K1077006
BBa_K1077006 Version 1 (Component)
nat fim switch b0034 GFP OFF orientation

BBa_K737048
BBa_K737048 Version 1 (Component)
The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42

BBa_K235025
BBa_K235025 Version 1 (Component)
[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.