BBa_K235025

BBa_K235025 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K235025

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kyliah Clarkson

Date created: 2009-09-02 11:00:00

Date modified: 2015-05-08 01:11:36

[K235022][K235000] (NAND gate, pAra and pLac input signal control, mCherry output signal)

Details

• Types: DnaRegion
• Roles: engineered_region; Project
• Sequences: BBa_K235025_sequence (Version 1)

Description

This BioBrick replicates the function of a NAND logic gate (http://en.wikipedia.org/wiki/NAND_gate). The inputs are a) an inducer for the lactose operon, and b) an inducer for the arabinose operon. The output is the production of the mCherry fluorescent protein (J06504).

IPTG in medium?	Arabinose as best carbon source?	mCherry produced?
no	no	yes
no	yes	yes
yes	no	yes
yes	yes	no

This system is sensitive to the cellular production of λcI repressor protein. Use with caution in cell strains with arabinose- or lactose-related sensitivity or insensitivity.

Notes

Constructed using three-antibiotic assembly. On plasmid pSB1A3.

Source

K235022+K235000

Components

Access	Instance	Definition
public	BBa_K235022	BBa_K235022
public	BBa_K235000	BBa_K235000

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K235022	1,3113	BBa_K235022
BBa_K235000	3122,3665	BBa_K235000

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K235025/1

Member of these Collections

• iGEM Parts Registry