BBa_K1159117
BBa_K1159117 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF3 version)

BBa_K1159118
BBa_K1159118 Version 1 (Component)
Red light triggered Kill Switch for plants translation unit (PhyB/PIF6 version)

BBa_K1613012
BBa_K1613012 Version 1 (Component)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.

BBa_T6002
BBa_T6002 Version 1 (Component)
test translational unit

BBa_K223032
BBa_K223032 Version 1 (Component)
trp-operon + RBS + GFP + TT makes sure trp-operon functions

BBa_K809108
BBa_K809108 Version 1 (Component)
Efficiency test of Q0255 Terminator

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

BBa_M36556
BBa_M36556 Version 1 (Component)
5' Bicistronic UTR (medium), does not include ATG start

BBa_K299106
BBa_K299106 Version 1 (Component)
xbaI G (xbaI site and additional G to behave like biobrick prefix after xba digest)

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

BBa_K290001
BBa_K290001 Version 1 (Component)
constitutive RhlR with bicistronic LuxI - GFP controlled by pRhl

BBa_M36291
BBa_M36291 Version 1 (Component)
Transcription terminator (apFAB391) (99% efficient)

BBa_K987001
BBa_K987001 Version 1 (Component)
This is a composite part which has the function to invert the temperature activation by the part: BB

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.