| Types | DnaRegion
|
| Roles | ribosome_entry_site
RBS
|
| Sequences | BBa_M36556_sequence (Version 1)
|
Description
5' UTR based on bicistronic junction architecture. First RBS is strong and drives expression of short leader cistron. Second RBS internal to first cistron control translation of gene of interest. Architecture is optimized so that downstream CDS for gene of interest does not have a chance to form a secondary structure with the 5' UTR. See BIOFAB.org for more information.
Notes
This specific RBS had a high expression of protein in whatever plasmid it was inserted in as observed from the data in http://io.biofab.org:5000/simple/bd_gois. It therefore guaranteed a high expression of our desired protein.
Source
The RBS was found in the biofab website ( http://io.biofab.org:5000/simple/bd_gois) under the tag BCD2.
