luxR-ndhBBa_K1036000 Version 1 (Component)lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
BBa_J69503BBa_J69503 Version 1 (Component)CI Repressible mRFP Reporter
BBa_K204069BBa_K204069 Version 1 (Component)rbs+cI repressor+terminater
BBa_J09044BBa_J09044 Version 1 (Component)strong RBS + cI
BBa_J09045BBa_J09045 Version 1 (Component)strong RBS + cI
BBa_J09048BBa_J09048 Version 1 (Component)weak RBS + cI
BBa_K1453304BBa_K1453304 Version 1 (Component)pBluescript II KS(+)_5_copy
BBa_K1453303BBa_K1453303 Version 1 (Component)pBluescript II KS(+)_3_copy
BBa_K1022129BBa_K1022129 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022128BBa_K1022128 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly
BBa_K1022127BBa_K1022127 Version 1 (Component)pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys
RBS-CI-TBBa_K079010 Version 1 (Component)CI protein with terminator and RBS
Strep-TagBBa_I757014 Version 1 (Component)Strep-Tag II affinity tag
BBa_K648031BBa_K648031 Version 1 (Component)RBS with cI repressor
BBa_K1104902BBa_K1104902 Version 1 (Component)pTet + RBS + cI + Term
BBa_J69502BBa_J69502 Version 1 (Component)CI-repressible mCherry Reporter
BBa_K1773008BBa_K1773008 Version 1 (Component)pLux/cI Right promoter with strong RBS
PL luxIBBa_K193001 Version 1 (Component)CI repressible LuxI enzyme generator
BBa_I9005BBa_I9005 Version 1 (Component)nor_input (tp901-cI) B0034.C0075
BBa_K091230BBa_K091230 Version 1 (Component)cI promoter+RBS+RFP+TT
BBa_I10200BBa_I10200 Version 1 (Component)434 cI repressor protein generator
BBa_K1647000BBa_K1647000 Version 1 (Component)CI operator site with GFP
PL GFPBBa_K193000 Version 1 (Component)GFP reporter regulated by CI.
BBa_K1053300BBa_K1053300 Version 1 (Component)PLtetO-1-RBS-cI-DT
BBa_K1421002BBa_K1421002 Version 1 (Component)plux R+RBS+cI+2TE
BBa_K909004BBa_K909004 Version 1 (Component)cI with strong ribosomal binding site
BBa_I719022BBa_I719022 Version 1 (Component)cI promoter with GFP reporter
BBa_K648032BBa_K648032 Version 1 (Component)Slightly weaker RBS with cI repressor
BBa_K648033BBa_K648033 Version 1 (Component)Very weak RBS with cI repressor
BBa_K318509BBa_K318509 Version 1 (Component)lacI pL + RBS + cI LVA + TT
BBa_K1707004BBa_K1707004 Version 1 (Component)TetR-ssRA repressor with the promoter of cI
BBa_K607037BBa_K607037 Version 1 (Component)pBAD/AraC generating cI transcription factor
BBa_K188222BBa_K188222 Version 1 (Component)RBS+cI repressor from phage 434 (+LVA)
BBa_K415011BBa_K415011 Version 1 (Component)PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI
BBa_K077039BBa_K077039 Version 1 (Component)cI under control of the plac promotor
BBa_J06570BBa_J06570 Version 1 (Component)Construction intermediate: 434 cI with RBS (B0034.C0052)
BBa_K145105BBa_K145105 Version 1 (Component)cI under T7 and P<sub>R</sub> dual promotor
BBa_K584011BBa_K584011 Version 1 (Component)Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP
PLac-LacY-BBa_I763013 Version 1 (Component)LacY and cI coding device switched on by IPTG
BBa_M31114BBa_M31114 Version 1 (Component)M13, gene II from HpaI to the end [1-831bp]
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K415005BBa_K415005 Version 1 (Component)pLux/cI-OR : RBS-mCherry : Term : p(tetR) : RBS-luxR : Term