BBa_K1647000
BBa_K1647000 Version 1 (Component)
CI operator site with GFP

BBa_J13101
BBa_J13101 Version 1 (Component)
LacI repressed lambda CI generator

PL GFP
BBa_K193000 Version 1 (Component)
GFP reporter regulated by CI.

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K1053300
BBa_K1053300 Version 1 (Component)
PLtetO-1-RBS-cI-DT

BBa_K909004
BBa_K909004 Version 1 (Component)
cI with strong ribosomal binding site

BBa_I719022
BBa_I719022 Version 1 (Component)
cI promoter with GFP reporter

BBa_K648032
BBa_K648032 Version 1 (Component)
Slightly weaker RBS with cI repressor

BBa_K648033
BBa_K648033 Version 1 (Component)
Very weak RBS with cI repressor

BBa_K318509
BBa_K318509 Version 1 (Component)
lacI pL + RBS + cI LVA + TT

Plamda-GFP
BBa_I763011 Version 1 (Component)
promoter lambda (cI regulated) with GFP (+LVA) reporter

BBa_K188222
BBa_K188222 Version 1 (Component)
RBS+cI repressor from phage 434 (+LVA)

BBa_K077039
BBa_K077039 Version 1 (Component)
cI under control of the plac promotor

BBa_K176017
BBa_K176017 Version 1 (Component)
pCI(R0051)(lambda CI-)->RBS+GFP+T

BBa_J06570
BBa_J06570 Version 1 (Component)
Construction intermediate: 434 cI with RBS (B0034.C0052)

BBa_K145105
BBa_K145105 Version 1 (Component)
cI under T7 and P_R dual promotor

BBa_K077019
BBa_K077019 Version 1 (Component)
pluxR with cI (lambda) behind a normal RBS

BBa_I3401
BBa_I3401 Version 1 (Component)
Lambda cI switch input device (B0034.C0051.B0015)

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K611017
BBa_K611017 Version 1 (Component)
cI Lambda Repressor and Promoter Wild Type Control

PLac-LacY-
BBa_I763013 Version 1 (Component)
LacY and cI coding device switched on by IPTG

BBa_M31114
BBa_M31114 Version 1 (Component)
M13, gene II from HpaI to the end [1-831bp]

BBa_I758601
BBa_I758601 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K145112
BBa_K145112 Version 1 (Component)
cI under T7 and PR_R dual promotor

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K177035
BBa_K177035 Version 1 (Component)
cI repressor from E. coli phage lambda (+LVA) under control of RBS.3 (medium)

BBa_K584008
BBa_K584008 Version 1 (Component)
Lambda cI and LuxR regulated hybrid promotor + RBS + MelA + RBS + AFP + term