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BBa_K611017

BBa_K611017 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K611017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Nicholas Csicsery

Date created: 2011-09-17 11:00:00

Date modified: 2015-05-08 01:12:53

cI Lambda Repressor and Promoter Wild Type Control

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K611017_sequence (Version 1)

Description

This part is can be used as a control for BBa_K611011 and BBa_K611014, which test either C0051 and R0051 mutants respectively.

Notes

There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.

Source

Built from existing parts in the registry though BioBrick assembly.

Components

Access	Instance	Definition
public public public public public public public	BBa_R0051 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0051	cI lam BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 cI lam

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0051 BBa_B0034 BBa_E0040 BBa_B0015 BBa_I13453 BBa_B0034 BBa_C0051	1,49 58,69 76,795 804,932 941,1070 1079,1090 1097,1871	cI lam BBa_B0034 GFP BBa_B0015 BBa_I13453 BBa_B0034 cI lam

Other Properties

igem#experience	None
igem#sampleStatus	It's complicated
igem#status	Planning
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K611017/1