BBa_K783028
BBa_K783028 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

T25 domain
BBa_K1088056 Version 1 (Component)
T25 domain of CyaA from Bordetella pertussis

BBa_K1968013
BBa_K1968013 Version 1 (Component)
PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_M31786
BBa_M31786 Version 1 (Component)
1st half of Gene III- preBamHI cut

BBa_K648103
BBa_K648103 Version 1 (Component)
RecA with mutation of Arg 243

BBa_K648102
BBa_K648102 Version 1 (Component)
RecA with mutation of Lys 286

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

PrtDEF
BBa_K258007 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_K1947015
BBa_K1947015 Version 1 (Component)
The test of the Pmsp3 promoter.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_K1725317
BBa_K1725317 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_I12026
BBa_I12026 Version 1 (Component)
Test of BBa_R0011 (LacI regulated) using YFP

BBa_J11009
BBa_J11009 Version 1 (Component)
variant of J11005 (low temp color) with stronger RBS

BBa_J14462
BBa_J14462 Version 1 (Component)
Composite part comprised of J13002 and J04650

BBa_K1051262
BBa_K1051262 Version 1 (Component)
The measurement pathway of degradation tag K1051208.

BBa_K1051260
BBa_K1051260 Version 1 (Component)
The measurement pathway of degradation tag K1051206.

BBa_K1051261
BBa_K1051261 Version 1 (Component)
The measurement pathway of degradation tag K1051207.

BBa_K783044
BBa_K783044 Version 1 (Component)
This is a MoClo converted version of BBa_J23108

BBa_J14458
BBa_J14458 Version 1 (Component)
Composite part comprised of J04500 and I13401

BBa_J14465
BBa_J14465 Version 1 (Component)
Composite part comprised of J13002 and I13401

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_K1676088
BBa_K1676088 Version 1 (Component)
Mutant 12 of LDH and wildtype LDP

BBa_K783045
BBa_K783045 Version 1 (Component)
This is a MoClo converted version of BBa_J23109

BBa_K783048
BBa_K783048 Version 1 (Component)
This is a MoClo converted version of BBa_B0031

BBa_K783043
BBa_K783043 Version 1 (Component)
This is a MoClo converted version of BBa_J23102

BBa_K783037
BBa_K783037 Version 1 (Component)
This is a MoClo converted version of BBa_J23118