BBa_K1968013

BBa_K1968013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1968013
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandra Adriana Schiavon Osorio
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-17 10:56:53

PgdaA constituve Phytobrick promoter: Glyceraldehyde-3-phosphate dehydrogenase from Aspergillus nige



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1968013_sequence (Version 1)

Description

Glyceraldehyde-3-phosphate dehydrogenase (GPDA) is an enzyme that catalyzes the sixth step of glycolysis. The gene gpdA is constitutively expressed in A. nidulans (Punt et al., 1990). Moreover, the gpdA promoter is able to drive robust transcription of heterologous genes. Intracellularly expressed model heterologous proteins like the E. coli b-galactosidase (lacZ) and b-glucuronidase (uiaA) under the control of the gpdA promoter can account for up to 10???25% of soluble protein in A. niger (Punt, Zegers, Busscher, Pouwels, & van den Hondel, 1991). The ability to drive constitutive tran- scription makes the gpdA promoter particularly useful for expression, under varying cultures conditions, of selection markers and reporter genes, many of which are from heterologous sources.

Notes

Phytrobrick format and MoClo compatible (Overhangs E and C)

Source

Genomic Sequence. The sequence was provided by the Fungal Stock Genetic Center. It was PCR out from plasmid P444 from their collection.

Sequence Annotation Location Component / Role(s)
Phytobrick Adapter Site E
Phytobrick Adapter Site C
PgdaA
1,4
678,681
5,677
feature/misc sequence_feature
sequence_feature feature/misc
promoter feature/promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1968013/1