BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K332024
BBa_K332024 Version 1 (Component)
A part of cell-cell-sigaling system

BBa_I13914
BBa_I13914 Version 1 (Component)
AiiA (+LVA) Tri-part (B0031.C0060.B0015)

BBa_I13911
BBa_I13911 Version 1 (Component)
AiiA (-LVA) Tri-part (B0034.C0160.B0015)

BBa_I13912
BBa_I13912 Version 1 (Component)
AiiA (+LVA) Tri-part (B0032.C0060.B0015)

BBa_I13910
BBa_I13910 Version 1 (Component)
AiiA (+LVA) Tri-part (B0034.C0060.B0015)

BBa_J14458
BBa_J14458 Version 1 (Component)
Composite part comprised of J04500 and I13401

BBa_J14465
BBa_J14465 Version 1 (Component)
Composite part comprised of J13002 and I13401

BBa_J14459
BBa_J14459 Version 1 (Component)
Composite part comprised of J04500 and J04630

BBa_J14464
BBa_J14464 Version 1 (Component)
Composite part comprised of J13002 and J04630

BBa_K1363006
BBa_K1363006 Version 1 (Component)
an experimental part used to test the conjugation

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K581003
BBa_K581003 Version 1 (Component)
SgrS2+Terminator (small RNA regulator, conjugate part of ptsG2)

BBa_K783053
BBa_K783053 Version 1 (Component)
This is a MoClo converted version of BBa_E0040

BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K783034
BBa_K783034 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K783051
BBa_K783051 Version 1 (Component)
This is a MoClo converted version of BBa_B0034

BBa_K783050
BBa_K783050 Version 1 (Component)
This is a MoClo converted version of BBa_B0033

BBa_K783038
BBa_K783038 Version 1 (Component)
This is a MoClo converted version of BBa_J23100

BBa_K896986
BBa_K896986 Version 1 (Component)
this is a gene about a T cell receptor

BBa_K1172914
BBa_K1172914 Version 1 (Component)
Part 2 of the Biosafety-System TetOR alive (TetO GFP)

BBa_K294205
BBa_K294205 Version 1 (Component)
This is a coding sequence of heat shock protein from E.coli

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.