BBa_K564016

BBa_K564016 Version 1

Component

This part has been discontinued.

Please consider using BBa_K564013 as a replacement.

Source:
http://parts.igem.org/Part:BBa_K564016
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Lewinska
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42

Upstream mutated chitoporin part fused with lacZ



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K564016_sequence (Version 1)

Description

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.In this biobrick we altered the binding site for ChiP regulator ??? ChiX. It was done in order to show that the regulatory system depends on the specific base pairing between ChiP and its regulator. The upstream part of this gene is fused with reporter gene lacZ to allow checking if the regulation works correctly.

Notes

There are no design considerations.

Source

The subparts were prepared: ChiP upstream part from Escherichia Coli W3110 genomic DNA by PCR and lacZ from part BBa_I732005 present in part registry.

igem#experience
None
 
igem#partStatus
Released HQ 2013
igem#sampleStatus
Discontinued
igem#status
Deleted
 
isReplacedBy
BBa_K564013/1
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K564016/1