Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anna Lewinska
Date created: 2011-09-19 11:00:00
Date modified: 2015-05-08 01:12:42
ChiP promoter and 5' UTR
RBS mutated AAGAGG-->AGGAGA
| Types | DnaRegion |
| Roles | sequence_feature DNA |
| Sequences | BBa_K564015_sequence (Version 1) |
Description
ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAa. We introduced the mutations in this sequence in the regulator binding site.Notes
There are no geding considereations.Source
It was obtained by PCR from Escherichia coli W3110 genomic DNA.| Sequence Annotation | Location | Component / Role(s) |
| ChiP mutated changed binding site for ChiX | 1,599 578,589 | CDS feature/cds sequence_alteration feature/mutation |