BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K112212
BBa_K112212 Version 1 (Component)
{rbs.int!} The integrase gene with native rbs site and stop codon, BBb format

BBa_K2082252
BBa_K2082252 Version 1 (Component)
RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI

xis
BBa_K112205 Version 1 (Component)
{rbs.xis!} The xis gene with native rbs site and stop codon; assembly standard 21

BBa_K598015
BBa_K598015 Version 1 (Component)
T7-promoter+TPP Up-regulated Hammerhead Ribozyme 1.20 with Native RBS+GFP+T7-terminator

BBa_K598016
BBa_K598016 Version 1 (Component)
T7-promoter+TPP Down-regulated Hammerhead Ribozyme 2.5 with Native RBS+GFP+T7-terminator

BBa_I742118
BBa_I742118 Version 1 (Component)
CrtZ with native ribosome binding site

rbs+crtY
BBa_I742155 Version 1 (Component)
crtY (lycopene cyclase) with native rbs

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K077041
BBa_K077041 Version 1 (Component)
AiiA and cII under control of plac promotor

rbs+crtE
BBa_I742152 Version 1 (Component)
crtE (geranylgeranyl pyrophosphate synthase) with native rbs.

BBa_I742149
BBa_I742149 Version 1 (Component)
sam8 (tyrosine ammonia lyase) with native rbs

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.