BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K2016006
BBa_K2016006 Version 1 (Component)
Superfolder GFP under control or medium constitutive promoter and RyhB siRNA domain

BBa_K750115
BBa_K750115 Version 1 (Component)
pBADcIT-PtetcIT-PcIGLT: arabinose or atc->cI protein->reduction of gfp(lva)

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K648130
BBa_K648130 Version 1 (Component)
Pr of Or tester with cI repressor with very weak RBS

BBa_K648129
BBa_K648129 Version 1 (Component)
Pr of Or tester with cI repressor with slightly weak RBS

BBa_K750112
BBa_K750112 Version 1 (Component)
GFP(lva) expression system controlled by cI or atc(RBS strength:1.0)

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G

BBa_K2082001
BBa_K2082001 Version 1 (Component)
Nanobody: Constant regions (insert variable regions to create your defined Nanobody or library)

BBa_K2123115
BBa_K2123115 Version 1 (Component)
Universal promoter (Tac + JK26) for both growth phase with downstream mer operator + K081014

BBa_K2144011
BBa_K2144011 Version 1 (Component)
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

BBa_I20292
BBa_I20292 Version 1 (Component)
There is no limit to what a man can do or where he can go if...

BBa_K2123116
BBa_K2123116 Version 1 (Component)
Universal promoter for both phase of growth in tandem with downstram mer operator + RFP (K081014)

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I733007
BBa_I733007 Version 1 (Component)
Weight cells either turn blue or die in response to both inputs and HSL level

BBa_I741109
BBa_I741109 Version 1 (Component)
Lambda Or operator region

BBa_K228013
BBa_K228013 Version 1 (Component)
(pSal PO) OR Gate - GFP

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.