Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:08:09
Coding sequence for Nuclease with His6 and LPXTG tag regulated by T7-promoter
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K2144011_sequence (Version 1) |
Description
Coding sequence for Nuclease from Staphylococcus Aureus, regulated by T7-promoter. Nuclease has the ability to cleave DNA. The inserted Linker Tag-sequence (BBa_K2144009) contains the gene encoding for His-tag which enables the use of IMAC to purify the combat protein. Also, it contains the LPTXG-tag. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortase is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif. The Linker in the Linker tag sequence enables for proper folding of the protein.Notes
To insert the Linker-tag sequence overhang PCR was performedSource
-| Access | Instance | Definition |
| public public | BBa_K2144000 BBa_K2144009 | Nuc-T7 BBa_K2144009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K2144000 BBa_K2144009 | 1,599 608,658 | Nuc-T7 BBa_K2144009 |