BBa_K2144009

BBa_K2144009 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2144009
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Oskar ??hman
Date created: 2016-10-11 11:00:00
Date modified: 2016-10-12 02:35:45

Sequence for His6-tag and LPXTGG-tag



Types
DnaRegion

Roles
tag

Tag

Sequences BBa_K2144009_sequence (Version 1)

Description

The His6-tag enables purification of proteins with IMAC. The LPXTG-tag enables the use of the "match making" enzyme sortase. The key feature of sortasee is the specific conjugation reaction it carries out, where the enzyme recognizes a specific amino acid sequence, a so called sorting motif (LPXTG motif in the case of S.aureus) and conjugate this sequence with another unit carrying an oligo glycine motif.

Notes

Coming soon

Source

Coming soon

Sequence Annotation Location Component / Role(s)
Linker
LPXTG
His6
1,14
15,31
32,48
sequence_feature feature/misc
feature/tag tag
tag feature/tag
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2144009/1