P-alsTBBa_K818300 Version 1 (Component)Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
BBa_K759001BBa_K759001 Version 1 (Component)Aggregation Module inducible by arabinose in E.coli.
BBa_K863020BBa_K863020 Version 1 (Component)bhal laccase from Bacillus halodurans with T7 promoter, RBS and His-tag
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Cello E. Coli PartsEco1C1G1T1_collection Version 1 (Collection)These are the Cello parts for E. Coli circuits
BBa_K098011BBa_K098011 Version 1 (Component)E. coli ompR, expressed at low level
BBa_K112245BBa_K112245 Version 1 (Component){pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid
BBa_K112246BBa_K112246 Version 1 (Component){pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid
BBa_K112247BBa_K112247 Version 1 (Component){pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid
BBa_K112248BBa_K112248 Version 1 (Component){pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid
RBS.TetRBBa_I15040 Version 1 (Component)RBS.TetR from pASKIBA3
BBa_K116601BBa_K116601 Version 1 (Component)HtlB (ftsH) coding region from E. coli
BBa_K112372BBa_K112372 Version 1 (Component){rbs.BgIII}{b1006}
BBa_K112130BBa_K112130 Version 1 (Component)Xis.Int with RBS, TrfA with RBS and terminator
BBa_K152006BBa_K152006 Version 1 (Component)frdBCD operon from E. coli (missing the first gene fumarate oxidase)
BBa_C0361BBa_C0361 Version 1 (Component)luxI (+LVA) - Codon-optimised for E.coli
BBa_C0378BBa_C0378 Version 1 (Component)lasI (+LVA) - Codon-optimised for E.coli
BBa_C0362BBa_C0362 Version 1 (Component)luxR - codon-optimised for E.coli
BBa_Z0301BBa_Z0301 Version 1 (Component)Venus YFP - E. coli codon optimized
BBa_J70029BBa_J70029 Version 1 (Component)RBS + mCherry gene, optimized for expression in Me. florum and E. coli
BBa_J70300BBa_J70300 Version 1 (Component)Strong Me. florum promoter
BBa_J70301BBa_J70301 Version 1 (Component)Heat shock inducible promoter for Me. florum
BBa_M10092BBa_M10092 Version 1 (Component){Pbad.rbs.prepro˃} For arabinose induced secretion
BBa_M10093BBa_M10093 Version 1 (Component){Pbad.rbs.prepro.StrepTag˃}
BBa_M10212BBa_M10212 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂upaG_short!}{dblTerm}
BBa_M10217BBa_M10217 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂TshA!}{dblTerm}
BBa_M10213BBa_M10213 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂Ag43_short!}{dblTerm}
BBa_M10215BBa_M10215 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{<AG4>}{<ehaB!]{dblTerm}
BBa_M10210BBa_M10210 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{AG4}{CPG_L6!}{dblTerm}
BBa_M10214BBa_M10214 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂espP(beta)!}{dblTerm}
BBa_M10216BBa_M10216 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{AG4}{CPompX!}{dblTerm}
BBa_M10225BBa_M10225 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂GS5-IILK˃}{˂TshA!}{dblTerm}
BBa_M10211BBa_M10211 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂AG4˃}{˂eCPX!}{dblTerm}
BBa_M10221BBa_M10221 Version 1 (Component){Pbad.rbs.prepro.StrepTag}}{˂GS5-IILK˃}{˂Ag43_short!}{dblTerm}
BBa_M10219BBa_M10219 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂GS5-IILKt˃} {˂eCPX!}{dblTerm}
BBa_M10223BBa_M10223 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{<GS5-IILK>}{<ehaB!]{dblTerm}
BBa_M10220BBa_M10220 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂GS5-IILK˃}{˂upaG_short!}{dblTerm}
BBa_M10218BBa_M10218 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPG_L6!}{dblTerm}
BBa_M10224BBa_M10224 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{GS5-IILK}{CPompX!}{dblTerm}
BBa_M10222BBa_M10222 Version 1 (Component){Pbad.rbs.prepro.StrepTag}{˂GS5-IILK˃}{˂espP(beta)!}{dblTerm}
BBa_K251000BBa_K251000 Version 1 (Component)coding sequene for E.coli heat shock protein hsp15 (example/ european meeting)
BBa_P101101BBa_P101101 Version 1 (Component)SAMT RBS.ORF.Term
BBa_P101102BBa_P101102 Version 1 (Component)BAMT RBS.ORF.Term
BBa_P101103BBa_P101103 Version 1 (Component)JMT RBS.ORF.Term (PCR)
BBa_P101104BBa_P101104 Version 1 (Component)JMT RBS.ORF.Term (Synth)
BBa_P101105BBa_P101105 Version 1 (Component)SAAT RBS.ORF.Term
BBa_P101106BBa_P101106 Version 1 (Component)BEAT RBS.ORF.Term
BBa_P101107BBa_P101107 Version 1 (Component)EHT1 RBS.ORF.Term