| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_J70029_sequence (Version 1)
|
Description
RBS + mCherry gene, optimized for expression in Me. florum and E. coli.
Notes
Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
Source
Synthesized by DNA 2.0, designed with Gene Designer. Initial tag of mCherry replaced with original AA sequence to eliminate the cryptic RBS + start internal to the coding sequence. Removed PvuII and Sau3AI sequences. Use of the compromise codon table guarantees no CGG codons and the use of TGG for tryptophan.
