BBa_K206008
BBa_K206008 Version 1 (Component)
pBAD reverse - terminator

BBa_K187057
BBa_K187057 Version 1 (Component)
ispU ORF reverse primer

BBa_K187323
BBa_K187323 Version 1 (Component)
kdsB ORF, Reverse Primer

C2/RGE
BBa_K1442105 Version 1 (Component)
C2 Testing Module (reverse GFP)

BBa_I741013
BBa_I741013 Version 1 (Component)
Reverse Total XylR Transciptional Regulator

BBa_J70459
BBa_J70459 Version 1 (Component)
yfp RBS, {0,5;15,10} family member - B0031 simulator (reverse oligo)

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.