BBa_K199170
BBa_K199170 Version 1 (Component)
T7 Promoter RBS RFP pLac CCACC (tRNA)

BBa_K199169
BBa_K199169 Version 1 (Component)
T7 Promoter RBS RFP pLac CUAGC (tRNA)

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K809710
BBa_K809710 Version 1 (Component)
GAL promoter + kozak + ZIM17 + T7 RNAP + ADH1 terminator

BBa_K779122
BBa_K779122 Version 1 (Component)
Short DNA reporter bottom strand (with ROX fluorophore) MammoBlock

BBa_K779121
BBa_K779121 Version 1 (Component)
Short DNA reporter top strand (with RQ quencher) MammoBlock

ChiP-lacZ
BBa_K564005 Version 1 (Component)
Chitoporin fused with lacZ -target for sRNA based regulation

PchiP-lacZ
BBa_K564002 Version 1 (Component)
Chitoporin fused with lacZ - target for sRNA based regulation

BBa_K199070
BBa_K199070 Version 1 (Component)
I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC

BBa_K1942000
BBa_K1942000 Version 1 (Component)
A shRNA corresponding DNA sequence for KRAS which could silence the gene

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K199071
BBa_K199071 Version 1 (Component)
I13453:K199014: Pbad promomtor with the suppressor tRNA of the codon AGGAC

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.