BBa_K658009BBa_K658009 Version 1 (Component)a bacteria population-control device with lux pR-3 driven by lacl+pL
BBa_K658011BBa_K658011 Version 1 (Component)a bacteria population-control device with lux pR-3/5 driven by lacl+pL
IBMc378IBMc378 Version 1 (Component)pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-M86(1-17)-PI-10aa-acVHH-T-cat-PBAD(SapI-)-RiboJ-BCD1-acVHH-10aa-M86(18-154)-S-mCherry*(193-236)-H6-T
BBa_K1366101BBa_K1366101 Version 1 (Component)Genetic construct for lpp gene deletion (C1)
luxR-ndhBBa_K1036000 Version 1 (Component)lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II
BBa_K195202BBa_K195202 Version 1 (Component)Lpp-OmpA-linker+alpha-V integrin gene+terminator
BBa_K2036028BBa_K2036028 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K2036027BBa_K2036027 Version 1 (Component)pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
BBa_K2036024BBa_K2036024 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K195203BBa_K195203 Version 1 (Component)RBS+Lpp-OmpA-linker+alpha-V integrin gene+terminator
BBa_K1231003BBa_K1231003 Version 1 (Component)Asr-64-Lpp-RBS-GFP is a dual-state construct
BBa_K145112BBa_K145112 Version 1 (Component)cI under T7 and PR<sub>R</sub> dual promotor
BBa_K1036003BBa_K1036003 Version 1 (Component)lux pL controlled luxR with lux pR controlled gfp (LVA-tag)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.