| Types | DnaRegion
|
| Roles | engineered_region
Device
|
| Sequences | BBa_K658009_sequence (Version 1)
|
Description
This device is made up of three subparts: a LuxI producer, a LuxR producer and a killer protein producer. It is designed to build a programmed bacterial death circuit, which is based on the quorum sensing system of Vibrio fischeri. iGEM-Team XMU-China has generated 7 bacteria population-control devices using RBSs of different strength and different lux pR mutants. This device has the ability to maintain cell density of the bacteria population at a relatively low value compared with bacteria without this circuit and extends the stable state. Lux pR-3(BBa_K658006) was used to regulate the expression of killer protein LacZalpha-ccdB.
Notes
This device is based on BBa_K658001. The mutated promoter lux pR-3 in the killer protein producer regulate the expression of killer protein LacZalpha-ccdB. The strength of promoters lux pR and its 3 mutants lux pR-3, lux pR-5, lux pR-3/5 were tested before they were used as regulators in a circuit. The strength of the lux pR-3 is related to the expression of the killer protein LacZalpha-ccdB. The higher strength a lux pR has, the lower cell density may be got in the steady state.
Source
BBa_K658001
