| Types | DnaRegion
|
| Roles | Regulatory
promoter
|
| Sequences | BBa_K658006_sequence (Version 1)
|
Description
The position 3 mutated promoter lux pR-3 is a product of site-directed mutagenesis of promoter lux pR (BBa_R0062). The promoter lux pR is based on the Vibrio fischeri quorum sensing gene promoters. The position 3 mutated promoter lux pR-3 gives a higher expression of downstream genes in an autoinduction system. In another word, the lux pR-3 has a higher efficiency than the lux pR. Similar to promoter lux pR, the expression is up-regulated by the action of LuxR protein combined with the signal molecule HSL.
Notes
In the promoter lux pR's sequence, the C residue of position 3 was changed to a T using PCR method.
Source
The position 3 mutated promoter lux pR-3 is based on promoter lux pR (BBa_R0062).
