Venus C
BBa_K537006 Version 1 (Component)
Venus C-Fusion

mFalpha
BBa_K1829006 Version 1 (Component)
Mating Factor Alpha (mFalpha)

BBa_K1333005
BBa_K1333005 Version 1 (Component)
M13 bacteriophage g8p with a FLAG tag on C-terminal

CBDcipA
BBa_K1615111 Version 1 (Component)
CBDcipA with N and C-terminal linker in RFC25

C-IgAP
BBa_K195410 Version 1 (Component)
C-terminal of IgA protease

BBa_K1321014
BBa_K1321014 Version 1 (Component)
CBDCipA with N and C-terminal linker

Thrombin c
BBa_K1362456 Version 1 (Component)
Thrombin recognition/cleavage site

BBa_K918000
BBa_K918000 Version 1 (Component)
Mating Factor Alpha Secretion Tag

BBa_I741018
BBa_I741018 Version 1 (Component)
Right facing promoter (for xylF) controlled by xylR and CRP-cAMP

BBa_K833000
BBa_K833000 Version 1 (Component)
Thermosensor with melting temperature of 35 to 37'C

SspDnaB(C)
BBa_K1362409 Version 1 (Component)
SspDnaB C-Intein cloning piece

BBa_K209006
BBa_K209006 Version 1 (Component)
Aar1 C-D part, Dd5P2 (aa435-787)

Intein C-t
BBa_K2075024 Version 1 (Component)
Intein and Intein Linker (C-term)

NpuDnaE(C)
BBa_K1362479 Version 1 (Component)
nonsplicing NpuDnaE C-Intein cloning piece

PompC(C)
BBa_K395301 Version 1 (Component)
PompC(C) Promoter regulated by phosphorylated OmpR

BBa_K115016
BBa_K115016 Version 1 (Component)
RNA thermometer (ROSE 27??C)

BBa_K209404
BBa_K209404 Version 1 (Component)
AarI C-D part, ActA (30-612)

BBa_K209410
BBa_K209410 Version 1 (Component)
AarI C-D part, ActA (255-392)

BBa_K392008
BBa_K392008 Version 1 (Component)
C. firmi beta-glucosidase (Silver standard compatible)

BBa_K1509000
BBa_K1509000 Version 1 (Component)
Coding for the trans-acting regulator SmtB

TerDnaE3(C
BBa_K1362405 Version 1 (Component)
TerDnaE3 C-Intein cloning piece

SspGyrB(C)
BBa_K1362407 Version 1 (Component)
SspGyrB C-Intein cloning piece

c-Myc
BBa_M0032 Version 1 (Component)
c-Myc affinity tag

SspDnaX(C)
BBa_K1362403 Version 1 (Component)
SspDnaX C-Intein cloning piece

com C
BBa_K233302 Version 1 (Component)
competence factor C , Acinetobacter BD413

YFP-C
BBa_K1616016 Version 1 (Component)
YC155 - C terminal YFP split

BBa_K209408
BBa_K209408 Version 1 (Component)
AarI C-D part, Intersectin (ITSN)

BBa_K209411
BBa_K209411 Version 1 (Component)
AarI C-D part, Lamellipodin (LPD) (775-1250)

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

BBa_K209015
BBa_K209015 Version 1 (Component)
Aar1 C-D part, Inp54p

BBa_K209409
BBa_K209409 Version 1 (Component)
AarI C-D part, Tuba

BBa_K115020
BBa_K115020 Version 1 (Component)
RNA thermometer (ROSE hairpin 37??C)

BBa_K209001
BBa_K209001 Version 1 (Component)
Aar1 C-D, PTEN (Dictyostelium discoideum), no STOP

C-IgAP
BBa_J32018 Version 1 (Component)
C-IgAP

BBa_K392014
BBa_K392014 Version 1 (Component)
C. josui CM10

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1871002
BBa_K1871002 Version 1 (Component)
Alpha amylase with mating factor alpha secretion

BBa_K331033
BBa_K331033 Version 1 (Component)
Tet Repressible C-terminal Arg Tagged ECFP (no terminator)

BBa_K392006
BBa_K392006 Version 1 (Component)
C. firmi CenA (Silver standard compatible)

BBa_K1159201
BBa_K1159201 Version 1 (Component)
Splitted and engineered C-terminal FbaB for isopeptide bound formation (SpyTag) in RFC[25]

BBa_K209413
BBa_K209413 Version 1 (Component)
AarI C-D part, Vav

BBa_K2012000
BBa_K2012000 Version 1 (Component)
c-di-GMP tandem riboswitche bc3-5

BBa_K106679
BBa_K106679 Version 1 (Component)
GFP, under pheromone-inducible promoter, 5' LexA Ops

c-Myc
BBa_T2008 Version 1 (Component)
c-Myc special internal domain (EQKLISEEDL)

BBa_K1491023
BBa_K1491023 Version 1 (Component)
C-terminus of T7 RNA Polymerase

c-Myc
BBa_T2007 Version 1 (Component)
c-Myc head domain (EQKLISEEDL)

BBa_J329002
BBa_J329002 Version 1 (Component)
Intermediate part for making a QS system

c-Myc
BBa_T2006 Version 1 (Component)
c-Myc tail domain (EQKLISEEDL)

eYFP_SH3li
BBa_K1602038 Version 1 (Component)
eYFP with C-terminal SH3 ligand and RBS BBa_B0034

BBa_K209010
BBa_K209010 Version 1 (Component)
Aar1 C-D part, RasC da (no CAAX) (Dictyostelium discoideum)