Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xenia Spencer-Milnes
Date created: 2014-10-14 11:00:00
Date modified: 2015-06-17 12:14:33
CBDCipA with N and C-terminal linker
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1321014_sequence (Version 1) |
Description
This CBD is from the Cellulosomal -scaffolding protein A (cipA) of Clostridium thermocellum including the endogenous linker sequences at the N and C-terminus (UniProt ID Q06851 link http://www.uniprot.org/uniprot/Q06851). It is in RFC25 format to allow for easy use in protein fusions.The CBD is part of the CBM3 family (link to cazy http://www.cazy.org/CBM3.html). This CBD has been used in many application: in fusions with cell adhesion peptides to enhance the properties of cellulose as a cell-growth matrix (Andrade et al 2010a, Andrade et al 2010b), fused to enzymes to remove contaminants from water (Kauffmann et al 2000) and fused to an antimicrobial peptide (Ramos, Domingues & Gama 2010).
At present the cloning for these constructs is still in progress to correct an illegal EcoRI site which was identified in the parts with this CBD. This can be achieved with a silent mutation via site-directed mutagenesis and we aim to send these parts to the registry once this is complete.
Notes
The DNA sequence from the relevant portion of http://www.ncbi.nlm.nih.gov/nuccore/144777 was codon optimised for E.coli. RFC[25] prefix and suffix were appended to the sequence with an additional 4 basepairs (gatc) at the beginning and end to allow space for the restriction enzymes to bind to the EcoRI and PstI sites at the ends of the sequence for cloning purposes.Source
The designed construct was ordered from as a GeneArt?? String (Invitrogen??? Life Technologies).| Sequence Annotation | Location | Component / Role(s) |
| Endogenous Linker (N-term) CBDcipA Endogenous Linker (C-term) | 1,126 127,603 604,711 | CDS feature/cds feature/cds CDS CDS feature/cds |